.
 

ÀÛ¼ºÀÏ : 22-07-15 16:17
20220722_Journal Club_ÀÓÈ£ÁØ
 ±Û¾´ÀÌ : °ü¸®ÀÚ
Á¶È¸ : 248  
1. A Dof-CLE circuit controls phloem organization
https://www.nature.com/articles/s41477-022-01176-0

2. Cell-by-cell dissection of phloem development links a maturation gradient to cell specialization
https://www.science.org/doi/10.1126/science.aba5531

3. Distinct mechanisms for genomic attachment of the 5¡¯ and 3¡¯ ends of Agrobacterium T-DNA in plants
https://www.nature.com/articles/s41477-022-01147-5

4. Cell size controlled in plants using DNA content as an internal scale
https://www.science.org/doi/10.1126/science.abb4348


¼±Á¤ ÀÌÀ¯:

1. Á¦°¡ ¿¬±¸ÇÏ°í ÀÖ´Â NAC020, NAC028, NAC057ÀÇ target Áß Çϳª°¡ BRX¶ó°í »ý°¢ÇÏ°í ÀÖ½À´Ï´Ù. ±×¸®°í BRX´Â BAM3¿Í CLE45¸¦ ¾ïÁ¦ÇÏ´Â ¿ªÇÒÀ» ÇÏ¿© phloem differentiation rate¸¦ Á¶ÀýÇÑ´Ù°í ¾Ë·ÁÁ® ÀÖ½À´Ï´Ù. ÀÌ ³í¹®¿¡¼­´Â ±× CLE25, 26, 45°¡ phloem¿¡¼­ ¹ßÇöµÇ´Â Dof-class Àü»ç ÀÎÀÚ¿¡ ÀÇÇØ Á¶ÀýµÈ´Ù´Â °ÍÀ» º¸¿©ÁÖ°í À־ Á¦ ¿¬±¸¿Í °ü·ÃÀÌ ÀÖ´Ù°í »ý°¢ÇÏ¿© ¼±Á¤ÇÏ¿´½À´Ï´Ù.

2. RootÀÇ phloem developmental trajectory¿¡ ´ëÇؼ­ single-cell data·Î ºÐ¼®ÇÏ¿© Á¤¸®ÇÏ¿´½À´Ï´Ù. ±×¸®°í phloem differentiation °úÁ¤¿¡¼­ PEAR¿Í PLETHORAÀÇ ¿ªÇÒÀ» ¿©·¯ transgenic line imagingÀ» È°¿ëÇÏ¿© º¸¿©ÁÖ¾ú±â¿¡ ¼±Á¤ÇÏ¿´½À´Ï´Ù.

3. Agrobacterium transfectionÀº plant genetic engineering Áß¿¡ Çϳª·Î ³Î¸® »ç¿ëµÇ´Â ¹æ¹ýÀ̸ç ÀúÈñ ½ÇÇè½Ç¿¡¼­µµ ³Î¸® »ç¿ëµÇ´Â ¹æ¹ýÀÔ´Ï´Ù. ÇÏÁö¸¸ AgrobacteriumÀÌ plant genome¿¡ T-DNA¿¡ integrationÇÏ´Â mechanism¿¡ ´ëÇؼ­´Â ¾ÆÁ÷ ¿ÏÀüÈ÷ ¹àÇôÁöÁö ¾Ê¾Ò½À´Ï´Ù. ÀÌ ³í¹®¿¡¼­´Â T-DNA integration mechanism °úÁ¤¿¡¼­ ½Ä¹° ´Ü¹éÁúÀÎ MRE11°ú TDP2°¡ °ü¿©ÇÔÀ» ¹àÇô³Â½À´Ï´Ù. T-DNA integration mechanismÀº Á¦°¡ Æò¼Ò¿¡µµ °ü½ÉÀÖ¾î ÇÏ´Â ³»¿ëÀÌ°í °ü·Ã ³í¹® Áß ÃÖ±Ù ³í¹®À̱⿡ ¼±Á¤Çß½À´Ï´Ù.

4. ÀÌ ³í¹®¿¡¼­´Â µþ¼¼Æ÷µéÀÇ Å©±â¸¦ G1 phase¿¡¼­ ¼­·Î °°°Ô ȸº¹½ÃÅ°´Âµ¥ °ü¿©ÇÏ´Â factor°¡ KRP4ÀÓÀ» È®ÀÎÇÏ¿´°í ±× mechanism¿¡ ´ëÇÑ ¸ðµ¨µµ Á¦½ÃÇÏ¿´½À´Ï´Ù. ÀÌ ³í¹®ÀÇ ½ÇÇèÀº shoot stem cell niche¿¡¼­ ÁøÇàÇÏ¿´½À´Ï´Ù. Á¦°¡ ¿¬±¸ÇÏ´Â protophloem development Áß division stage¿¡¼­´Â cell size°¡ ´Þ¶óÁö±â ¶§¹®¿¡ cell size Á¶Àý°ú °ü·ÃµÈ ³í¹®À» ã°Ô µÇ¾ú°í ÀÌ ³í¹®À» ¼±Á¤ÇÏ°Ô µÇ¾ú½À´Ï´Ù.